Human bACE1 (Beta-Site APP Cleaving Enzyme 1) ELISA Kit
Sizes: 48T, 96T
Catalogue Numbers: ELK2029-48, ELK2029-96
Citations, Manuals and MSDS Available upon request.
Alternative Names: ASP2; Beta Secretase; Memapsin 2; Aspartyl protease 2; Beta-site amyloid precursor protein cleaving enzyme 1; Membrane-associated aspartic protease 2
Species: Human
Assay Type: Sandwich
Sensitivity: 0.11 ng/mL
Standard: 16 ng/mL
Detection Range: 0.25-16 ng/mL
Sample Type: Serum, Plasma, cerebrospinal fluid and other Biological Fluids
Assay length: 3.5h
Research Areas: Neuroscience
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human bACE1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human bACE1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human bACE1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human bACE1 in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: P56817
Research Use Only.