Human BDH1 (3-Hydroxybutyrate Dehydrogenase 1) ELISA Kit
Sizes: 48T, 96T, 96T*5
Catalogue Numbers: ELK4147-48, ELK4147-96, ELK4147-5x96
Citations, Manuals and MSDS Available upon request.
Alternative Names: DBH; SDR9C1; D-beta-Hydroxybutyrate Dehydrogenase,Mitochondrial; Short Chain Dehydrogenase/Reductase Family 9C,Member 1
Assay Type: Sandwich
Sensitivity: 0.057 ng/mL
Standard: 10 ng/mL
Detection Range: 0.16-10 ng/mL
Sample Type: Tissue homogenates, cell lysates and other biological fluids.
Assay Length: 3.5h
Research Areas: Enzyme and Kinase
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human BDH1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human BDH1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human BDH1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human BDH1 in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: Q02338
Research Use Only