Human BMP4 (Bone Morphogenetic Protein 4) Microsample ELISA Kit
Sizes: 48T, 96T, 96T*5
Catalogue Numbers: ELK7500MS-48, ELK7500MS-96, ELK7500MS-5x96
Citations, Manuals and MSDS Available upon request.
Alternative Names: BMP2B; BMP2-B; BMP2B1; ZYME; DVR4; Bone morphogenetic protein 2B
Assay Type: Sandwich
Sensitivity: 12.6 pg/mL
Standard: 2000 pg/mL
Detection Range: 31.25-2000 pg/mL
Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length: 3.5h
Research Areas: Developmental science; Bone metabolism;
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human BMP4. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human BMP4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human BMP4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human BMP4 in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: P12644
Research Use Only