Human BRCA1 (Breast Cancer Susceptibility Protein 1) ELISA Kit
Sizes: 48T, 96T, 96T*5
Catalogue Numbers: ELK1904-48, ELK1904-96, ELK1904-5x96
Citations, Manuals and MSDS Available upon request.
Alternative Names: BRCAI; BRCC1; IRIS; PSCP; RNF53; Breast Cancer 1,Early Onset; RING finger protein 53
Assay Type: Sandwich
Sensitivity: 0.067 ng/mL
Standard: 10 ng/mL
Detection Range: 0.16-10 ng/mL
Sample Type: Tissue homogenates, cell lysates and other biological fluids
Assay Length: 3.5h
Research Areas: Signal transduction; Tumor immunity;
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human BRCA1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human BRCA1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human BRCA1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human BRCA1 in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: P38398
Research Use Only