Human C4d (Complement Component 4d) ELISA Kit
Sizes: 48T, 96T, 96T*5
Catalogue Numbers: ELK6431-48, ELK6431-96, ELK6431-5x96
Citations, Manuals and MSDS Available upon request.
Alternative Names: C4d
Assay Type: Sandwich
Sensitivity: 0.96 ng/mL
Standard: 140 ng/mL
Detection Range: 2.19-140 ng/mL
Sample Type: serum, plasma and other biological fluids
Assay Length: 3.5h
Research Areas: Signal transduction; Infection immunity; Hematology;
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human C4d. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human C4d. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human C4d, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human C4d in the samples is then determined by comparing the OD of the samples to the standard curve.
Research Use Only