Human C5a (Complement Component 5a) ELISA Kit
Sizes: 24T, 48T, 96T
Catalogue Numbers: E-EL-H0190-24T, E-EL-H0190-48T, E-EL-H0190-96T
Citations, Manuals and MSDS Available upon request.
Abbreviation: C5a
UNIProt ID: P01031
Target Synonyms: C5a
Research Areas: Cell Biology, Metabolism, Immunology, Signal transduction
Target Species: Human
Application: ELISA
Usage: This ELISA kit applies to the in vitro quantitative determination of Human C5a concentrations in serum, plasma and other biological fluids.
Tested Sample Types: Serum, Plasma and other biological fluids
Type: Sandwich
Assay Time: 3.5h
Detection Type: Colormetric
Sensitivity: 46.88 pg/mL
Detection Range: 78.13-5000 pg/mL
Test Principle: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human C5a. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human C5a and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human C5a, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human C5a. You can calculate the concentration of Human C5a in the samples by comparing the OD of the samples to the standard curve.
Cross Reactivity: This kit recognizes Human C5a in samples. No significant cross-reactivity or interference between Human C5a and analogues was observed
Recovery Rate: 80%-120%
Precision: Both intra-CV and inter-CV are < 10%.
Storage: 2-8℃/-20℃, 12 months
Shipping: ice bag