Human CEA (Carcinoembryonic Antigen) ELISA Kit
Sizes: 24T, 48T, 96T
Catalogue Numbers: E-OSEL-H0016-24, E-OSEL-H0016-48, E-OSEL-H0016-96
Citations, Manuals and MSDS Available upon request.
Abbreviation: CEA
UNIProt ID: P06731
Target Synonyms: CEA; Carcinoembryonic Antigen;
Research Areas: Cancer, Immunology, Stem cells, Tags and Cell Markers
Target Species: Human
Application: ELISA
Usage: This ELISA kit applies to the in vitro quantitative determination of Human CEA concentrations in serum, plasma.
Tested Sample Types: Serum, Plasma
Type: Sandwich
Assay Time: 1.5h
Detection Type: Colormetric
Sensitivity: 0.47 ng/mL
Detection Range: 0.78-50 ng/mL
Test Principle: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human CEA. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Human CEA are added to the micro ELISA plate wells. Human CEA in samples (or standards) combines with the coated antibody and HRP linked detection antibody special to Human CEA.Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450±2 nm. The OD value is proportional to the concentration of Human CEA. The concentration of Human CEA in the samples is then determined by comparing the OD of the samples to the standard curve.
Cross Reactivity: This kit recognizes Human CEA in samples. No significant cross-reactivity or interference between Human CEA and analogues was observed
Recovery Rate: 80%-120%
Precision: Both intra-CV and inter-CV are < 10%.
Storage: 2-8℃, 6 months
Shipping: ice bag