Human F13B (Coagulation Factor XIII B Polypeptide) ELISA Kit
Sizes: 48T, 96T, 96T*5
Catalogue Numbers: ELK10620-48, ELK10620-96, ELK10620-5x96
Citations, Manuals and MSDS Available upon request.
Alternative Names: F13-B; FXIIIB; Fibrin-stabilizing factor B subunit; Protein-glutamine gamma-glutamyltransferase B chain; Transglutaminase B chain
Assay Type: Competitive Inhibition
Sensitivity: 1.24 ng/mL
Standard: 200 ng/mL
Detection Range: 3.13-200 ng/mL
Sample Type: plasma
Assay Length: 2h
Research Areas: Signal transduction; Metabolic pathway; Hematology;
Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human F13B. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human F13B. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human F13B in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: P05160
Research Use Only