Human GIF (Gastric Intrinsic Factor) ELISA Kit
Sizes: 48T, 96T, 96T*5
Catalogue Numbers: ELK3212-48, ELK3212-96, ELK3212-5x96
Citations, Manuals and MSDS Available upon request.
Alternative Names: TCN3; IF; IFMH; INF; Transcobalamin III; Vitamin B Synthesis
Assay Type: Competitive Inhibition
Sensitivity: 0.91 ng/mL
Standard: 200 ng/mL
Detection Range: 3.13-200 ng/mL
Sample Type: Serum, plasma and other biological fluids
Assay Length: 2h
Research Areas: Endocrinology;
Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human GIF. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human GIF. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human GIF in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: P27352
Research Use Only