Human Gly (Glycine) ELISA Kit
Sizes: 48T, 96T, 96T*5
Catalogue Numbers: ELK0836-48, ELK0836-96, ELK0836-5x96
Citations, Manuals and MSDS Available upon request.
Alternative Names: Glycine
Assay Type: Competitive Inhibition
Sensitivity: 0.56 µg/mL
Standard: 100 µg/mL
Detection Range: 1.57-100 µg/mL
Sample Type: serum, plasma, cell lysates, cell culture supernates and other biological fluids
Assay Length: 2h
Research Areas: Microbiology and biochemistry
Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human Gly. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human Gly. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human Gly in the samples is then determined by comparing the OD of the samples to the standard curve.
Research Use Only