Human GT (Gastrin) ELISA Kit
Sizes: 48T, 96T, 96T*5
Catalogue Numbers: ELK1814-48, ELK1814-96, ELK1814-5x96
Citations, Manuals and MSDS Available upon request.
Alternative Names: GAST; GAS; Gastrin component I; Big gastrin; Gastrin component II; Gastrin component III; Gastrin 17; Gastrin 34
Assay Type: Competitive Inhibition
Sensitivity: 4.21 pg/mL
Standard: 1000 pg/mL
Detection Range: 15.63-1000 pg/mL
Sample Type: Serum, plasma, cell lysates, cell culture supernates and other biological fluids
Assay Length: 2h
Research Areas: Metabolic pathway; Endocrinology; Gastroenterology; Hormone metabolism;
Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human GT. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human GT. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human GT in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: P01350
Research Use Only