Human HB (Hemoglobin) ELISA Kit
Sizes: 48T, 96T, 96T*5
Catalogue Numbers: ELK1093-48, ELK1093-96, ELK1093-5x96
Citations, Manuals and MSDS Available upon request.
Alternative Names: Hgb; Haemoglobin; Heterotetramer (αβ)2
Assay Type: Competitive Inhibition
Sensitivity: 2.6 µg/mL
Standard: 500 µg/mL
Detection Range: 7.82-500 µg/mL
Sample Type: serum, plasma, erythrocyte lysates, cell culture supernates and other biological fluids
Assay Length: 2h
Research Areas: Metabolic pathway; Infection immunity; Hematology; Neuroscience;
Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human HB. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human HB. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human HB in the samples is then determined by comparing the OD of the samples to the standard curve.
Research Use Only