Human HBd (Hemoglobin Delta) ELISA Kit
Sizes: 48T, 96T, 96T*5
Catalogue Numbers: ELK5336-48, ELK5336-96, ELK5336-5x96
Citations, Manuals and MSDS Available upon request.
Alternative Names: Delta-globin
Assay Type: Competitive Inhibition
Sensitivity: 0.91 µg/mL
Standard: 200 µg/mL
Detection Range: 3.13-200 µg/mL
Sample Type: Serum, plasma, erythrocyte lysates and other biological fluids
Assay Length: 2h
Research Areas: Hematology;
Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human HBd. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human HBd. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human HBd in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: P02042
Research Use Only