Human hs-CRP (high sensitivity C Reactive Protein) ELISA Kit
Sizes: 48T, 96T, 96T*5
Catalogue Numbers: ELK8534-48, ELK8534-96, ELK8534-5x96
Citations, Manuals and MSDS Available upon request.
Alternative Names: C-RP; PTX1; Pentraxin-Related; Pentraxin 1; hs-CRP
Assay Type: Sandwich
Sensitivity: 0.5 ng/mL
Standard: 100 ng/mL
Detection Range: 1.57-100 ng/mL
Sample Type: serum, plasma, tissue homogenates, cell lysates, urine, cerebrospinal fluid, cell culture supernates and other biological fluids
Assay Length: 3.5h
Research Areas: Tumor immunity; Infection immunity; Cardiovascular biology; Rheumatology;
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human hs-CRP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human hs-CRP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human hs-CRP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human hs-CRP in the samples is then determined by comparing the OD of the samples to the standard curve.
Research Use Only