Human HS (Heparan Sulfate) ELISA Kit
Sizes: 48T, 96T, 96T*5
Catalogue Numbers: ELK0768-48, ELK0768-96, ELK0768-5x96
Citations, Manuals and MSDS Available upon request.
Alternative Names: HS; Heparan Sulfate
Assay Type: Competitive Inhibition
Sensitivity: 51.9 ng/mL
Standard: 8000 ng/mL
Detection Range: 125-8000 ng/mL
Sample Type: serum, plasma, cell lysates, cell culture supernates and other biological fluids
Assay Length: 2h
Research Areas: Signal transduction; Metabolic pathway; Tumor immunity; Infection immunity; Developmental science;
Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human HS. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human HS. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human HS in the samples is then determined by comparing the OD of the samples to the standard curve.
Research Use Only