Human IAPP (Islet Amyloid Polypeptide) ELISA Kit
Sizes: 48T, 96T, 96T*5
Catalogue Numbers: ELK4831-48, ELK4831-96, ELK4831-5x96
Citations, Manuals and MSDS Available upon request.
Alternative Names: IAP; DAP; Amylin; Insulinoma Amyloid Polypeptide; Diabetes-associated peptide
Assay Type: Competitive Inhibition
Sensitivity: 2.47 pg/mL
Standard: 500 pg/mL
Detection Range: 7.82-500 pg/mL
Sample Type: Serum, plasma, cell lysates, cell culture supernates and other biological fluids
Assay Length: 2h
Research Areas: Metabolic pathway; Endocrinology; Hepatology;
Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human IAPP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human IAPP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human IAPP in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: P10997
Research Use Only