Human IL-6 (Interleukin 6) ELISA Kit
Sizes: 24T, 48T, 96T
Catalogue Numbers: E-OSEL-H0001-24, E-OSEL-H0001-48, E-OSEL-H0001-96
Citations, Manuals and MSDS Available upon request.
Abbreviation: IL-6
UNIProt ID: P05231
Target Synonyms: Interleukin 6; IL6; BSF2; HGF; HSF; IFNB2; CDF;
Research Areas: Cancer, Metabolism, Immunology, Neuroscience
Target Species: Human
Application: ELISA
Usage: This ELISA kit applies to the in vitro quantitative determination of Human IL-6 concentrations in serum, plasma.
Tested Sample Types: Serum, Plasma
Type: Sandwich
Assay Time: 1.5h
Detection Type: Colormetric
Sensitivity: 1.02 pg/mL
Detection Range: 3.13-200 pg/mL
Test Principle: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human IL-6. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Human IL-6 are added to the micro ELISA plate wells. Human IL-6 in samples (or standards) combines with the coated antibody and HRP linked detection antibody special to Human IL-6.Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450±2 nm. The OD value is proportional to the concentration of Human IL-6. The concentration of Human IL-6 in the samples is then determined by comparing the OD of the samples to the standard curve.
Cross Reactivity: This kit recognizes Human IL-6 in samples. No significant cross-reactivity or interference between Human IL-6 and analogues was observed
Recovery Rate: 80%-120%
Precision: Both intra-CV and inter-CV are < 10%.
Storage: 2-8℃, 6 months
Shipping: ice bag