Human KLK1 (Kallikrein 1) ELISA Kit
Sizes: 48T, 96T, 96T*5
Catalogue Numbers: ELK10611-48, ELK10611-96, ELK10611-5x96
Citations, Manuals and MSDS Available upon request.
Alternative Names: KLKR; Klk6; HK1; Kallikrein 1,Renal/Pancreas/Salivary; Kidney/pancreas/salivary gland kallikrein; Tissue kallikrein
Assay Type: Competitive Inhibition
Sensitivity: 0.12 ng/mL
Standard: 20 ng/mL
Detection Range: 0.32-20 ng/mL
Sample Type: Serum, plasma and other biological fluids
Assay Length: 2h
Research Areas: Enzyme and Kinase; Tumor immunity; Hepatology;
Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human KLK1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human KLK1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human KLK1 in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: P06870
Research Use Only