Human MIF (Macrophage Migration Inhibitory Factor) ELISA Kit
Sizes: 24T, 48T, 96T
Catalogue Numbers: E-EL-H6170-24, E-EL-H6170-48, E-EL-H6170-96
Citations, Manuals and MSDS Available upon request.
Abbreviation: MIF
UNIProt ID: P14174
Target Synonyms: MIF
Research Areas: Cancer, Immunology
Target Species: Human
Application: ELISA
Usage: This ELISA kit applies to the in vitro quantitative determination of Human MIF concentrations in serum, plasma and other biological fluids.
Tested Sample Types: Serum, Plasma and other biological fluids
Type: Sandwich
Assay Time: 3.5h
Detection Type: Colormetric
Sensitivity: 18.75 pg/mL
Detection Range: 31.25-2000 pg/mL
Test Principle: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human MIF. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human MIF and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human MIF, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human MIF. You can calculate the concentration of Human MIF in the samples by comparing the OD of the samples to the standard curve.
Cross Reactivity: This kit recognizes Human MIF in samples. No significant cross-reactivity or interference between Human MIF and analogues was observed
Recovery Rate: 80%-120%
Precision: Both intra-CV and inter-CV are < 10%.
Storage: 2-8℃/-20℃, 12 months
Shipping: ice bag