Human NKX2-5 (NK2 Homeobox 5) ELISA Kit
Sizes: 48T, 96T, 96T*5
Catalogue Numbers: ELK0416-48, ELK0416-96, ELK0416-5x96
Citations, Manuals and MSDS Available upon request.
Alternative Names: Cardiac-specific homeobox; Homeobox protein CSX; Homeobox protein NK-2 homolog E; CSX; NKX2.5; NKX2E
Assay Type: Sandwich
Sensitivity: 21.4 pg/mL
Standard: 5000 pg/mL
Detection Range: 78.13-5000 pg/mL
Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length: 3.5h
Research Areas: Signal transduction
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human NKX2-5. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human NKX2-5. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human NKX2-5, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human NKX2-5 in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: P52952
Research Use Only