Human ucOC (Undercarboxylated Osteocalcin) ELISA Kit
Sizes: 48T, 96T
Catalogue Numbers: ELK9383-48, ELK9383-96
Citations, Manuals and MSDS Available upon request.
Alternative Names: ucOC
Species: Human
Assay Type: Sandwich
Sensitivity: 0.059 ng/mL
Standard: 10 ng/mL
Detection Range: 0.16-10 ng/mL
Sample Type: Serum, Plasma, Tissue Homogenates
Assay length: 3.5h
Research Areas: Metabolic pathway; Endocrinology; Hormone metabolism; Bone metabolism;
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human ucOC. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human ucOC. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human ucOC, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human ucOC in the samples is then determined by comparing the OD of the samples to the standard curve.
Research Use Only.