Mouse CD74 (HLA class II histocompatibility antigen gamma chain) ELISA Kit
Sizes: 48T, 96T
Catalogue Numbers: ELK0926-48, ELK0926-96
Citations, Manuals and MSDS Available upon request.
Alternative Names: CD74; HLA class II histocompatibility antigen gamma chain; CLIP; DHLAG; HLADG; Ii; II; MHC HLA-DR gamma chain; p33; Ia-gamma; INVG34; CD74 antigen; HLA-DR antigens-associated invariant chain; HLA-DR-gamma; Ia antigen-associated invariant chain; Ia-associated invariant chain
Species: Mouse
Assay Type: Sandwich
Sensitivity: 28.1 pg/mL
Standard: 5000 pg/mL
Detection Range: 78.13-5000 pg/mL
Sample Type: Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates and other Biological Fluids
Assay length: 3.5h
Research Areas: Immunology
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse CD74. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse CD74. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse CD74, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse CD74 in the samples is then determined by comparing the OD of the samples to the standard curve.
Research Use Only.