Rat KYN (Kynurenine) ELISA Kit
Sizes: 48T, 96T
Catalogue Numbers: ELK9738-48, ELK9738-96
Citations, Manuals and MSDS Available upon request.
Alternative Names: KYN, Kynurenine
Species: Rat
Assay Type: Sandwich
Sensitivity: 3.5 pmol/ml
Standard: 500 pmol/ml
Detection Range: 7.82-500 pmol/ml
Sample Type: serum, plasma, tissue homogenates, saliva, cell lysates, follicular fluid and other biological fluids
Assay Length: 3.5h
Research Areas: Metabolism
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat KYN. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat KYN. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat KYN, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat KYN in the samples is then determined by comparing the OD of the samples to the standard curve.
UNIProt ID: -
Research Use Only