Rat MBL1 (Mannose-binding protein A) ELISA Kit
Sizes: 48T, 96T
Catalogue Numbers: ELK9752-48, ELK9752-96
Citations, Manuals and MSDS Available upon request.
Alternative Names: MBL1, Mbl1Mannose-binding protein A, MBP-A, Mannan-binding protein
Species: Rat
Assay Type: Sandwich
Sensitivity: 0.059 ng/mL
Standard: 10 ng/mL
Detection Range: 0.16-10 ng/mL
Sample Type: Serum, plasma and other biological fluids
Assay Length: 3.5h
Research Areas: Signal Transduction, Cancer
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat MBL1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat MBL1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat MBL1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat MBL1 in the samples is then determined by comparing the OD of the samples to the standard curve.
UNIProt ID: P19999
Research Use Only