Rat VAP-1 (VascularAdhesion Protein 1) ELISA Kit
Sizes: 48T, 96T
Catalogue Numbers: ELK9634-48, ELK9634-96
Citations, Manuals and MSDS Available upon request.
Alternative Names: AOC3 , HPAO, SSAO, Amine oxidase, copper containing 3
Species: Rat
Assay Type: Sandwich
Sensitivity: 0.10 ng/mL
Standard: 10 ng/mL
Detection Range: 0.16-10 ng/mL
Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length: 3.5h
Research Areas: Neuroscience, Signal Transduction
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat VAP-1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat VAP-1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat VAP-1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat VAP-1 in the samples is then determined by comparing the OD of the samples to the standard curve.
UNIProt ID: O08590
Research Use Only