Simian OC/BGP (Osteocalcin) ELISA Kit
Sizes: 48T, 96T
Catalogue Numbers: ELK9627-48, ELK9627-96
Citations, Manuals and MSDS Available upon request.
Alternative Names: BGLAP, BGP, OC, OCN, bone gamma-carboxyglutamate protein, Osteocalcin
Species: Simian
Assay Type: Sandwich
Sensitivity: 0.47 ng/mL
Standard: 50 ng/mL
Detection Range: 0.78-50 ng/mL
Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length: 3.5h
Research Areas: Stem Cells, Signal Transduction,
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Simian OC/BGP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Simian OC/BGP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Simian OC/BGP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Simian OC/BGP in the samples is then determined by comparing the OD of the samples to the standard curve.
UNIProt ID: P02819
Research Use Only