Native Bovine Deoxyribonuclease I
Size: 100 U
Catalogue Number: PHAM-266
Citations, Manuals and MSDS Available upon request.
Product Description: DNase I is an endonuclease that acts on phosphodiester bonds adjacent to pyrimidines to produce polynucleotides with terminal 5′-phosphates. In the presence of Mg2+, DNAse I cleaves each strand of DNA independently and the cleavage sites are random. Both DNA strands are cleaved at approximately the same site in the presence of Mn2+.2 The pH optimum is found to be between 7 and 8. Divalent cations such as Mn2+, Ca2+, Co2+, and Zn2+ are activators of the enzyme.3 A concentration of 5 mM Ca2+ stabilizes the enzyme against proteolytic digestion. DNAse I from bovine pancreas consists of four chromatographically distinguishable components, A, B, C, and D, with molar ratios being 4:1:1 respectively. Only minor amounts of D are found.4 2-Mercaptoethanol, chelators, sodium dodecyl sulfate (SDS)5 and actin6 are known to inhibit the enzyme activity.
Storage: −20°C
Applications: Used for the removal of DNA from protein samples.
Activity: ≥ 2,000 U/mL
CAS#: 9003-98-9
Research Use Only