Native Clostridium histolyticum Collagenase
Size: 100 mg
Catalogue Number: DIGS-253
Citations, Manuals and MSDS Available upon request.
Product Description: Collagenases are endopeptidases that digest native collagen in the triple helix region. Collagens are the major fibrous component of animal extracellular connective tissue. Bacterial collagenases differ from vertebrate collagenases in that they exhibit broader substrate specificity (Peterkofsky 1982, Birkedal-Hansen 1987). Unlike animal collagenases that split collagen in its native triple-helical conformation (Woolley et al. 1975, Gross et al. 1974), bacterial collagenase is unique because it can degrade both water-insoluble native collagens and water-soluble denatured ones. It can attack almost all collagen types, and is able to make multiple cleavages within triple helical regions (Mookhtiar and Van Wart 1992).
Storage: 2-8°C
Applications: Isolation of adipocytes, hepatocytes, and cells from lung, epithelium and adrenal tissue Isolation of cardiomyocytes and cells from bone, cartilage, muscle, thyroid, and endothelium Isolation of mammary and various other soft tissues Isolation of human and porcine pancreatic islet cells (Kin 2007) Treatment of tissues with crude collagenase, with its mixture of proteolytic activities, provides gentle, selective digestion of the intercellular matrix with little damage to cells or loss of viability AFA collagenase is suitable for applications needing to avoid introduction of animal derived pathogens into bioprocessing procedures
Activity: Type 1 > 125 units per mg; Type 2 > 125 units per mg; Type 3 > 100 units per mg; Type 4 > 160 units per mg; Type 5 > 450 units per mg
CAS#: 2593923
Research Use Only