Native Microorganism P-hydroxybenzoate Hydroxylase
Size: 5 KU
Catalogue Number: DIA-203
Citations, Manuals and MSDS Available upon request.
Product Description: In enzymology, a 4-hydroxybenzoate 3-monooxygenase (EC 1.14.13.2) is an enzyme that catalyzes the chemical reaction: 4-hydroxybenzoate + NADPH + H+ + O2 ↔ protocatechuate + NADP+ + H2O. The 4 substrates of this enzyme are 4-hydroxybenzoate, NADPH, H+, and O2, whereas its 3 products are protocatechuate, NADP+, and H2O. This enzyme belongs to the family of oxidoreductases, specifically those acting on paired donors, with O2 as oxidant and incorporation or reduction of oxygen. The oxygen incorporated need not be derived from O2 with NADH or NADPH as one donor, and incorporation of one atom o oxygen into the other donor. This enzyme participates in benzoate degradation via hydroxylation and 2,4-dichlorobenzoate degradation. It employs one cofactor, FAD.
Storage: -20°C
Applications: This enzyme is useful for enzymatic determination of choline esterase when coupled with protocatechuate 3, 4-dioxygenase.
Activity: GradeⅢ 20U/mg-solid or more (containing approx. 40% of stabilizers)
CAS#: 9059-23-8
Research Use Only