PerCP-Cy5.5 Anti-Human CD274/PD-L1 Antibody (29E.2A3)
Sizes: 20T, 100T
Catalogue Numbers: PCP55-30044-20, PCP55-30044-100
Citations, Manuals and MSDS Available upon request.
Target: Human CD274
Form: Liquid
Conjugation: PerCP-Cy5.5
Conjugation Information: PerCP-Cy5.5 is designed to be excited by the blue laser (488 nm) and detected using an optical filter centered near 675 nm (e.g., a 690/50 nm bandpass filter).
Host: Mouse
Clonality: Monoclonal
Clone ID: 29E.2A3
Isotype: IgG2b, κ
Storage: PBS with 0. 1% sodium azide, 1%BSA, pH 7.3, 2-8℃ for 12 months (Avoid repeated freeze / thaw cycles.)
Background: CD274, also known as PD-L1 and B7-H1, is type I transmembrane glycoprotein that serves as a ligand for CD279 (PD-1). This interaction is believed to regulate the balance between the stimulatory and inhibitory signals needed for responses to microbes and maintenance of self-tolerance. CD274 is involved in the costimulation of T cell proliferation and IL-10 and IFN-γ production in an IL-2-dependent and CD279-independent manner. Conflicting data has shown that CD274 can inhibit T cell proliferation and cytokine production, and alternatively, enhance T cell activation. Other studies suggest that CD274 may signal bidirectionally, raising interesting implications for its expression in a wide variety of cell types, including T and B cells, antigen-presenting cells, and nonhematopoietic cells.
Synonyms: B7H1PD-L1PDCD1L1PDCD1LG1PDL1|B7 homolog 1|B7-H|B7-H1|Programmed cell death ligand 1
Uniprot ID: Q9NZQ7
Reactivity: Human
Tested Application: FC
Recommended Dilution: Volume per test: 5μL. Each lot of this antibody is quality control tested by Flow cytometryometric analysis. The amount of the reagent is suggested to be used 5 µL of antibody per test (million cells in 100 µL staining volume or per 100 µL of whole blood). Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use.
Gene ID: 29126
Category: Flow cytometryometry Antibodies
Research Use Only