Chicken Polyclonal Antibody to Human GFRA-1
Size: 100 µg
Catalogue Number: 303-100
Citations, Manuals and MSDS Available upon request.
Immunogen: Human GFRa-1
Immunogen Description: Recombinant His-tagged human GFRa-1 protein produced using CHO-based Icosagen Cell factory Ltd. proprietary suspension cell line. For production of hGFRa-1, glycosylphosphatidyl-inositol GPI-anchor was removed and protein was secreted to the cell culture supernatant. Protein was purified by Ni-affinity chromatography following gel-filtration from cell culture supernatant
Background: Glial cell line-derived neurotrophic factor (GDNF) and neurturin (NTN) are two structurally related, potent neurotrophic factors that play key roles in the control of neuron survival and differentiation. The GFRa-1 is a member of the GDNF receptor family. It is a glycosylphosphatidylinositol (GPI)-linked cell surface receptor for both GDNF and NTN, and mediates activation of the RET tyrosine kinase receptor (www.ncbi.nlm.nih.gov/gene/2674)
UniProt ID: P56159
Alternative Names: RET ligand 1, TGF-beta related neurotrophic factor receptor 1
Clonality: Chicken polyclonal
Class: IgY
Reactivity: Human GFRa-1 doesn’t cross-react with hGFRa-2, hGFRa-3, hGFRa-4
Application: ELISA, WB, IF
Protocol: ELISA: 0.02 to 0.05 µg/ml; WB 0.1 to 0.2 µg/ml; IF 0.5 to 1 µg/ml
Polyclonal antibody working titer has to be established practically for each particular antigen and assay format
Purification: GFRa-1 affinity purified
Buffer: Concentrated ammonium sulphate in PBS pH 7.4
Shipping: This product is shipped in non-frozen liquid form in ambient conditions
Storage: Store at +4 °C upon receipt. As product is (NH4)2SO4 precipitate, mix well by pipetting or vortexing prior use
Research Use Only