Chicken Polyclonal Antibody to Human GFRA-2
Size: 100 µg
Catalogue Number: 304-100
Citations, Manuals and MSDS Available upon request.
Immunogen: Human GFRa-2
Immunogen Description: Recombinant His-tagged human GFRα-2 protein produced using CHO-based Icosagen Cell factory Ltd. proprietary suspension cell line. For production of hGFRα-2, glycosylphosphatidyl-inositol GPI-anchor was removed and protein was secreted to the cell culture supernatant. Protein was purified by Ni-affinity chromatography following gel-filtration from cell culture supernatant
Background: Glial cell line-derived neurotrophic factor (GDNF) and neurturin (NTN) are two structurally related, potent neurotrophic factors that play key roles in the control of neuron survival and differentiation. GFRa-2 is a member of the GDNF receptor family. It is a glycosylphosphatidyl-inositol(GPI)-linked cell surface receptor for both GDNF and NTN, and mediates activation of the RET tyrosine kinase receptor. This encoded protein acts preferentially as a receptor for NRTN compared to its other family member, GDNF family receptor alpha 1. http://www.ncbi.nlm.nih.gov/gene/2675
UniProt ID: O00451
Alternative Names: GDNF receptor beta, Neurturin receptor alpha, RET ligand 2 TGF-beta-related neurotrophic factor receptor 2
Clonality: Chicken polyclonal
Class: IgY
Reactivity: Human GFRa-2, doesn’t cross-react with hGFRa-1, hGFRa-3, hGFRa-4
Application: ELISA, WB, IF
Protocol: WB 0.2 to 1 µg/ml; ELISA: 6.25 to 8 ng/ml; IF 1 to 2 µg/ml
Polyclonal antibody working titer has to be established practically for each particular antigen and assay format
Purification: GFRa-2 affinity purified
Buffer: Concentrated ammonium sulphate in PBS pH 7.4
Shipping: This product is shipped in non-frozen liquid form in ambient conditions
Storage: Store at +4°C upon receipt. As product is (NH4)2SO4 precipitate, mix well by pipetting or vortexing prior use
Research Use Only