Chicken Polyclonal Antibody to Human GFRA-3
Size: 100 µg
Catalogue Number: 305-100
Citations, Manuals and MSDS Available upon request.
Immunogen: Human GFRa-3
Immunogen Description: Recombinant His-tagged human GFRa-3 protein produced using CHO-based Icosagen Cell factory Ltd. proprietary suspension cell line. For production of hGFRa-3, glycosylphosphatidyl-inositol GPI-anchor was removed and protein was secreted to the cell culture supernatant. Protein was purified by Ni-affinity chromatography following gel-filtration from cell culture supernatant
Background: The GFRa-3 is a glycosylphosphatidylinositol(GPI)-linked cell surface receptor and a member of the GDNF receptor family. It forms a signaling receptor complex with RET tyrosine kinase receptor and binds the ligand, artemin
UniProt ID: O60609
Alternative Names: GDNF receptor alpha-3
Clonality: Chicken polyclonal
Class: IgY
Reactivity: Human GFRa-3, doesn’t cross-react with hGFRa-1, hGFRa-2, hGFRa-4
Application: ELISA, WB, IF
Protocol: WB 1 to 2 µg/ml; ELISA: 3 to 6.25 ng/ml; IF 0.3 to 0.67 µg/ml.
Polyclonal antibody working titer has to be established practically for each particular antigen and assay format
Purification: GFRα-3 affinity purified
Buffer: Concentrated ammonium sulphate in PBS pH 7.4
Shipping: This product is shipped in non-frozen liquid form in ambient conditions
Storage: Store at +4°C upon receipt. As product is (NH4)2SO4 precipitate, mix well by pipetting or vortexing prior use
Research Use Only