Mouse Monoclonal Antibody to Bovine Papillomavirus Type 1 E2-Protein
Size: 100 µg
Catalogue Number: A1-154-100
Citations, Manuals and MSDS Available upon request.
Immunogen: BPV type 1 E2
Immunogen Description: Recombinant full length BPV type 1 E2 protein purified from E. coli
Background: E2 protein plays a central role in the viral life cycle as it regulates both transcription and replication of the viral genome. Can either activate or repress transcription. The E1-E2 protein complex binds to the origin of DNA replication activating replication of the BPV
UniProt ID: P03122
Clonality: Mouse monoclonal
Clone: 5H4
Class: IgG
Reactivity: Bovine, BPV type 1 E2 protein, mapped to 310-410
Application: ELISA, IF
Protocol: ELISA 0.1 to 0.2 µg/ml; IF 0.33 to 20 µg/ml
This is a conformational antibody and does not work in Western immunoblotting analysis or in other denaturative conditions.
Induces DNA/protein complex supershift in mobility shift assay.
Monoclonal antibody working titer has to be established practically for each particular antigen and assay format.
Purification: Protein A purification
Buffer: PBS with 50% glycerol
Shipping: This product is shipped in non-frozen liquid form in ambient conditions
Storage: Store at – 20 or -70 °C upon receipt. Divide antibody into aliquots prior usage. Avoid multiple freeze-thaw cycles as product degradation may result
Research Use Only