Mouse Monoclonal Antibody to HCV Subtype 1B NS5B Protein
Size: 100 µg
Catalogue Number: A2-427-100
Citations, Manuals and MSDS Available upon request.
Immunogen: HCV 1b NS5B
Immunogen Description: Recombinant Hepatitis C Virus subtype 1b nonstructural protein 5B (NS5B) RNA-dependent RNA polymerase (RdRp)
Background: Non-structural protein 5B (NS5B) represents the RNA-dependent RNA polymerase (RdRp) of Hepatitis C Virus, which is a small positive strand RNA virus in the family Flaviviridae. HCV is a major causative agent of acute and chronic hepatitis, hepatocellular carcinoma and liver cirrhosis. The single subunit RNA-dependent RNA polymerase is absolutely essential for the viral replication.
UniProt ID: Q9WMX2
Clonality: Mouse monoclonal
Clone: 7F12
Class: IgG1
Reactivity: Human, HCV subtype 1b NS5B, Epitope mapped to amino acids 77-86 (TVKAKLLSVE)
Application: ELISA, WB, IP
Protocol: WB in reduced conditions 1 µg/ml;
Optimal conditions for IP should be determined for each particular application.
Monoclonal antibody working titer has to be established practically for each particular antigen and assay format.
Purification: Protein G purification
Buffer: PBS pH 7.4, with 0.1% sodium azide
Shipping: This product is shipped in non-frozen liquid form in ambient conditions
Storage: Store at - 20 to -70 °C upon receipt. Divide antibody into aliquots prior usage. Avoid multiple freeze-thaw cycles as product degradation may result.
Research Use Only