Mouse Monoclonal Antibody to Hev B1 (Rubber Elongation Factor, Ref)
Size: 100 µg
Catalogue Number: A3-701-100
Citations, Manuals and MSDS Available upon request.
Immunogen: Hev b1
Immunogen Description: Hev b1-MBP fusion protein expressed and purified from E. coli.
Background: Liquid latex from the rubber tree, Hevea brasiliensis, is the source of natural rubber latex (NRL) and contains over 200 proteins; 14 of them have been identified as allergens. Only some allergens retain their allergenic properties through the manufacturing processes. The NRL allergens that have been shown to be clinically relevant to genuine NRL allergy, and present in the final NRL products with maintained allergenicity are Hev b1, Hev b3, Hev b5 and Hev b6.02.
UniProt ID: P15252
Alternative Names: Rubber elongation factor protein – REF (Hevea brasiliensis)
Clonality: Mouse monoclonal
Clone: 45662
Class: IgG1
Reactivity: Recombinant and native Hev b1
Application: ELISA, WB
Protocol: ELISA 1:32 000. Reacts as binding antibody in capture ELISA with Hev b1 detection monoclonal antibody A3-700-100. WB 1:2000 to 1:4000
Monoclonal antibody working titer has to be established practically for each particular antigen and assay format
Purification: Protein G purification
Buffer: PBS, 0.1% sodium azide
Shipping: This product is shipped in non-frozen liquid form in ambient conditions
Storage: Store at - 20 to -70 °C upon receipt. Divide antibody into aliquots prior usage. Avoid multiple freeze-thaw cycles as product degradation may result
Research Use Only