α-Ketoglutarate Dehydrogenase (α-KGDH) Activity Assay Kit
Size: 96T
Catalogue Number: E-BC-K083-M-96
Citations, Manuals and MSDS Available upon request.
Target Synonym: α-KGDH
Research Areas: Mitochondria Function;Tricarboxylic Acid（TCA）Cycle
Result Type: Enzyme Activity
Detection Instrument: Microplate reader
Tested Sample Types: serum, plasma, animal and plant tissue
Assay Time: 40 min
Detection Type: Colorimetric method
Sensitivity: 8.3 U/L
Detection Range: 8.3-42.3 U/L
Test Principle: α-ketoglutarate dehydrogenase (α-KGDH) is a key enzyme in the tricarboxylic acid (TCA) cycle. While the substrate being catalyzed by α-KGDH, NAD+ is reduced to NADH, and electrons are transfered to WST-8 under the action of hydrogen transmitter to produce yellow product. The activity of α-KGDH can be calculated by measuring the change of absorbance value at 450 nm
Inter CV: 8.5
Intra CV: 1.5
Storage: This product can be stored at -20°C for 12 months with shading light.
Shipping: Ice bag