β-Hydroxybutyrate (Ketone Body) Colorimetric Assay Kit
Sizes: 48T, 96T
Catalogue Numbers: E-BC-K785-M-48, E-BC-K785-M-96
Citations, Manuals and MSDS Available upon request.
Target Synonym: β-HB
Research Areas: Metabolic Diseases;Glycolysis and Lipid Metabolism
Result Type: Quantitative
Detection Instrument: Microplate reader
Tested Sample Types: serum, plasma, urine, animal tissue
Assay Time: 60 min
Detection Type: Colorimetric method
Sensitivity: 0.01 mmol/L
Detection Range: 0.01-2.00 mmol/L
Test Principle: β- hydroxybutyrate dehydrogenase can catalyze the oxidative dehydrogenation of β-HB. Meanwhile, NAD+ is reduced to NADH, Which under the action of hydrogen transmitter, transfer electrons to WST-8 to produce the yellow product. The content of β-HB can be calculated by measuring the change of absorbance value at 450 nm.
Inter CV: 4
Intra CV: 2
Storage: This product can be stored at 2~8°C for 12 months with shading light.
Shipping: Ice bag