Acetyl-CoA Colorimetric Assay Kit
Sizes: 48T, 96T
Catalogue Numbers: E-BC-K652-M-48, E-BC-K652-M-96
Citations, Manuals and MSDS Available upon request.
Target Synonym: Acetyl-CoA
Research Areas: Tricarboxylic Acid（TCA）Cycle;Mitochondria Function
Result Type: Quantitative
Detection Instrument: Microplate reader
Tested Sample Types: animal tissue
Assay Time: 15 min
Detection Type: Colorimetric method
Sensitivity: 150 nmol/mL
Detection Range: 150-500 nmol/mL
Test Principle: Malate and NAD+ form oxoacetate and NADH under the action of malate dehydrogenase, then acetyl-coa and oxoacetate form coenzyme A and citrate under the action of citrate synthetase. The content of acetyl-coA can be calculated by measuring the change of absorbance value at 340 nm
Inter CV: 5.3
Intra CV: 4
Storage: This product can be stored at -20°C for 12 months with shading light.
Shipping: Ice bag