Acid Phosphatase (ACP) Activity Assay Kit
Sizes: 96T, 500 Assays
Catalogue Numbers: E-BC-K094-M-96, E-BC-K094-M-500
Citations, Manuals and MSDS Available upon request.
Target Synonym: ACP
Research Areas: Amino Acids and Proteins
Result Type: Enzyme Activity
Detection Instrument: Microplate reader
Tested Sample Types: Serum, plasma, hydrothorax, urine, cells, cell culture supernatant, animal tissue
Assay Time: 45 min
Detection Type: Colorimetric method
Sensitivity: 1.40 U/100 mL
Detection Range: 1.40-40 U/100 mL
Test Principle: Acid phosphatase decomposes disodium phenyl phosphate under acidic conditions to produce free phenol and phosphoric acid. Phenol acts with 4-aminoantipyrine in alkaline solution, and oxidizes to a derivative of red quinone by potassium ferricyanide. The activity of the ACP can be calculated by measuring the OD value at 520 nm.
Inter CV: 9.4
Intra CV: 2.1
Storage: This product can be stored at 2~8°C for 12 months with shading light.
Shipping: Ice bag