Adenosine Deaminase (ADA) Activity Assay Kit
Sizes: 48T, 96T, 500 Assays
Catalogue Numbers: E-BC-K197-M-48, E-BC-K197-M-96, E-BC-K197-M-500
Citations, Manuals and MSDS Available upon request.
Target Synonym: ADA
Research Areas: Liver and Renal Function
Result Type: Enzyme Activity
Detection Instrument: Microplate reader
Tested Sample Types: serum, plasma, animal tissue
Assay Time: 40 min
Detection Type: Colorimetric method
Sensitivity: 0.03 U/L
Detection Range: 0.03-99 U/L
Test Principle: Adenosine deaminase (ADA) can hydrolyzed the substrate adenosine to form hypoxanthine riboside, which is hydrolyzed by purine riboside phosphatase to produce hypoxanthine and phosphate ribose. Under the action of xanthine oxidase, hypoxanthine produces hydrogen peroxide, which produces red substance under the action of peroxidase, 4-aminotepyrine and color source. The red substance has the maximum absorption peak at 550 nm and the changes of absorbance is proportional to the activity of ADA.
Inter CV: 6
Intra CV: 3
Storage: This product can be stored at 2~8°C for 12 months with shading light.
Shipping: Ice bag