Alanine Aminotransferase (ALT/GPT) Activity Assay Kit
Sizes: 48T, 96T, 500 Assays
Catalogue Numbers: E-BC-K235-M-48, E-BC-K235-M-96, E-BC-K235-M-500
Citations, Manuals and MSDS Available upon request.
Target Synonym: ALT;GPT
Research Areas: Liver and Renal Function
Result Type: Enzyme Activity
Detection Instrument: Microplate reader
Tested Sample Types: Serum, plasma, animal tissue, cells, cell culture supernatant
Assay Time: 80 min
Detection Type: Colorimetric method
Sensitivity: 0.75 IU/L
Detection Range: 0.75-72.3 IU/L
Test Principle: ALT catalyze the amino conversion reaction between alanine and α-ketoglutaric acid to produce pyruvic acid and glutamic acid at pH 7.4 and 37℃. Then phenylhydrazine was added to form phenylhydrazone with pyruvic acid. Phenylhydrazone is reddish brown under alkaline conditions. ALT activity can be calculated by measuring the OD values at 510 nm.
Inter CV: 9
Intra CV: 5.3
Storage: This product can be stored at 2~8°C for 12 months with shading light.
Shipping: Ice bag