Aspartate Aminotransferase (AST/GOT) Activity Assay Kit
Sizes: 48T, 96T, 500 Assays
Catalogue Numbers: E-BC-K236-M-48, E-BC-K236-M-96, E-BC-K236-M-500
Citations, Manuals and MSDS Available upon request.
Target Synonym: AST;GOT
Research Areas: Liver and Renal Function
Result Type: Enzyme Activity
Detection Instrument: Microplate reader
Tested Sample Types: Serum, plasma, animal tissue, cells, cell culture supernatant
Assay Time: 80 min
Detection Type: Colorimetric method
Sensitivity: 1.1 IU/L
Detection Range: 1.1-72.3 IU/L
Test Principle: AST/GOT enables alpha-ketoglutaric acid and aspartic acid to displace amino and keto groups to form glutamic acid and oxaloacetic acid. Oxaloacetic acid can decarboxylate itself to form Pyroracemic acid during the reaction. Pyroracemic acid reacted with 2,4-dinitrophenylhydrazine (DNPH) to form 2,4, dinitrophenylhydrazone showing reddish brown in alkaline solution. Measure the OD values and calculate the enzyme activity.
Inter CV: 6.8
Intra CV: 5.3
Storage: This product can be stored at 2~8°C for 12 months with shading light.
Shipping: Ice bag