
Aspartate Aminotransferase (AST/GOT) Activity Assay Kit
Size: 100 Assays
Catalogue Number: E-BC-K236-S-100
Citations, Manuals and MSDS Available upon request.
Target Synonym: AST;GOT
Research Areas: Liver and Renal Function
Result Type: Enzyme Activity
Detection Instrument: Spectrophotometer
Tested Sample Types: Serum, plasma, animal tissue
Assay Time: 100 min
Detection Type: Colorimetric method
Sensitivity: 0.38 IU/L
Detection Range: 0.38-72.30 IU/L
Test Principle: AST enables alpha-ketoglutaric acid and aspartic acid to displace amino to form glutamic acid and oxaloacetic acid. Oxaloacetic acid can decarboxylate itself to form Pyroracemic acid during the reaction. Pyroracemic acid reacted with 2,4-dinitrophenylhydrazine (DNPH) to form 2,4-dinitrophenylhydrazone showing reddish brown in alkaline solution. Measure the OD values and calculate the enzyme activity.
Inter CV: 4.9
Intra CV: 4.2
Storage: This product can be stored at 2~8°C for 12 months with shading light.
Shipping: Ice bag