BCA Protein Colorimetric Assay Kit
Sizes: 96T, 500 Assays
Catalogue Numbers: E-BC-K318-M-96, E-BC-K318-M-500
Citations, Manuals and MSDS Available upon request.
Target Synonym: TP
Research Areas: Amino Acids and Proteins
Result Type: Quantitative
Detection Instrument: Microplate reader
Tested Sample Types: Serum, plasma, cell culture supernatant, tissue, cells
Assay Time: 40 min
Detection Type: Colorimetric method
Sensitivity: 0.0165 mg/mL
Detection Range: 0.0165-1 mg/mL
Test Principle: Cu2+ can be reduced to Cu+ by protein in alkaline condition. Cu+ can combine with BCA reagent and form purple complex, which has a maximum absorption peak at 562 nm. The absorbance value is proportional to the protein concentration. Therefore, the protein concentration can be calculated according to the OD value.
Inter CV: 4.5
Intra CV: 2.2
Storage: This product can be stored at room temperature for 12 months with shading light.
Shipping: Ice bag