Bradford Protein Colorimetric Assay Kit
Sizes: 96T, 500 Assays
Catalogue Numbers: E-BC-K168-M-96, E-BC-K168-M-500
Citations, Manuals and MSDS Available upon request.
Target Synonym: TP
Research Areas: Amino Acids and Proteins;Liver and Renal Function
Result Type: Quantitative
Detection Instrument: Microplate reader
Tested Sample Types: Serum, plasma, animal tissue and cell
Assay Time: 20 min
Detection Type: Colorimetric method
Sensitivity: 0.046 mg/mL
Detection Range: 0.046-0.6 mg/mL
Test Principle: Coomassie brilliant blue G-250 is red under the free state, and it has the maximum absorbance at 465 nm. When the Coomassie brilliant blue G-250 combined to protein, the compound will have the maximum at 595 nm. The absorbance value is directly proportional to the protein content, so the concentration of total protein can be calculated directly by measuring the OD value at 595 nm.
Inter CV: 8.2
Intra CV: 3.2
Storage: This product can be stored at 2~8°C/-20°C for 12 months with shading light. Please refer to the manual for the specific storage condition of the components.
Shipping: Ice bag