Choline Acetyltransferase (ChAT) Activity Assay Kit (Tissue Samples)
Sizes: 48T, 96T, 500 Assays
Catalogue Numbers: E-BC-K125-M-48, E-BC-K125-M-96, E-BC-K125-M-500
Citations, Manuals and MSDS Available upon request.
Target Synonym: ChAT
Research Areas: Amino Acids and Proteins
Result Type: Enzyme Activity
Detection Instrument: Microplate reader
Tested Sample Types: Animal tissue
Assay Time: 65 min
Detection Type: Colorimetric method
Sensitivity: 1.21 U/g fresh weigh
Detection Range: 1.21-40 U/g fresh we
Test Principle: Acetyl-CoA can react with choline under the catalysis of choline acetyltransferase (ChAT) to produce coenzyme A (CoA) , CoA can combine with the 4, 4-dithiopyridine. The activity of ChAT can be calculated indirectly by measuring the OD value at 324 nm.
Inter CV: 9.4
Intra CV: 5.1
Storage: This product can be stored at 2~8°C/-20°C for 12 months with shading light. Please refer to the manual for the specific storage condition of the components.
Shipping: Ice bag