Creatinine (Cr) Colorimetric Assay Kit (Sarcosine Oxidase Method)
Sizes: 48T, 96T, 500 Assays
Catalogue Numbers: E-BC-K188-M-48, E-BC-K188-M-96, E-BC-K188-M-500
Citations, Manuals and MSDS Available upon request.
Target Synonym: Cr
Research Areas: Liver and Renal Function
Result Type: Quantitative
Detection Instrument: Microplate reader
Tested Sample Types: Serum, plasma, urine
Assay Time: 30 min
Detection Type: Colorimetric method
Sensitivity: 3.8 μmol/L
Detection Range: 20.45-800 μmol/L
Test Principle: Creatinine (Cr) can be catalyzed by creatinase and generates creatine. Creatine can be hydrolyzed into sarcosine and urea by creatinase. The sarcosine can be catalyzed by sarcosine oxidase and form glycine, formaldehyde and hydrogen peroxide. The reaction between hydrogen peroxide, 2,4- (6-Tri-iodine-3- hydroxybenzoic acid) and 4-ampyrone can be catalyzed by peroxidase and form pink compound. Creatinine content can be calculated indirectly by measuring the OD value at 515 nm.
Inter CV: 3.7
Intra CV: 1.4
Storage: This product can be stored at 2~8°C for 12 months with shading light.
Shipping: Ice bag