D-Lactic Acid/Lactate (LA) Colorimetric Assay Kit
Sizes: 48T, 96T, 500 Assays
Catalogue Numbers: E-BC-K002-M-48, E-BC-K002-M-96, E-BC-K002-M-500
Citations, Manuals and MSDS Available upon request.
Target Synonym: D-LA
Research Areas: Glycolysis and Lipid Metabolism
Result Type: Quantitative
Detection Instrument: Microplate reader
Tested Sample Types: Serum, plasma, animal tissue, saliva sample
Assay Time: 20 min
Detection Type: Colorimetric method
Sensitivity: 0.06 mmol/L
Detection Range: 0.06-8.0 mmol/L
Test Principle: Using NAD+ as H+ receptor, D-lactate dehydrogenase (LDH) catalyzes the reaction of D-lactic acid and NAD+ to generate pyruvic acid and NADH respectively. NBT is reduced to a kind of purple compound during the reaction. Measure the OD value at 530 nm, and the concentration of D-lactic acid can be calculated.#N/A
Inter CV: 7.7
Intra CV: 3.8
Storage: This product can be stored at 2~8°C for 12 months with shading light.
Shipping: Ice bag