Glutaminase (GLS) Activity Assay Kit
Size: 96T
Catalogue Number: E-BC-K660-M-96
Citations, Manuals and MSDS Available upon request.
Target Synonym: GLS
Research Areas: Amino Acids and Proteins
Result Type: Enzyme Activity
Detection Instrument: Microplate reader
Tested Sample Types: serum (plasma) , animal, plant tissue
Assay Time: 80min
Detection Type: Colorimetric method
Sensitivity: 0.003 U/L
Detection Range: 0.003-18.0 U/L
Test Principle: Glutamine is decomposed to produce glutamic acid under the action of glutaminase. Glutamic acid is further transformed by glutamic acid dehydrogenase. Meanwhile, NAD+ is reduced to NADH, Which under the action of hydrogen transmitter, transfer electrons to WST-8 to produce the yellow product. The activity of glutaminase can be calculated by measuring the change of absorbance value at 450 nm.
Inter CV: 8.3
Intra CV: 5
Storage: This product can be stored at -20°C for 12 months with shading light.
Shipping: Ice bag